P-180 Hyaluronidase used in oocyte denudation inhibits the proliferation and the viability of cumulus cells through the inhibition of midkine

Abstract Study question Does hyaluronidase (HAse) have possible negative or positive effects on cumulus cells (CCs) during oocyte denudation before ICSI by altering midkine (MK) levels? Summary answer Hyaluronidase inhibits the proliferation of CCs via inhibition MK activity which may result in poor embryo quality and low success. What is known already abundantly expressed ovarian follicles. secreted from cumulus-granulosa that surround oocytes was shown to promote cytoplasmic maturation oocytes. This effect could be mediated its' anti-apoptotic some other mechanisms. The removal (OD) done order select grade ICSI. HAse applied for break down hyaluronic acid, present at high levels cumulus-oocyte complex OD. To date, there only been a few reported cases toxic design, size, duration study prospective, randomized with 90 healthy women between September 2017 2018. Participants/materials, setting, methods diagnosed as male factor ages 21 40 who underwent cultured concentrations 0.1 IU/ml, 1 10 IU/ml. apoptosis indices (Flow cytometry), structures [Transmissive electron microscopy (TEM)], (ELISA) were evaluated every 24 hours 48 hours. One way-Anova used p < 0.05 considered statistically significant. Main results role chance application all decreased cell numbers (p 0.05). highest decrease number viability apoptotic detected 48th hour concentration caused end Limitations, reasons caution aged below above not evaluated. Therefore, overall resistance fragility these In addition, should female infertility such PCOS. Wider implications findings first report examine activity. 2-3 lines are commonly left protect stress-induced reactions. means resulting loss competence. Trial registration Tthe Ethics Committee Directive Non-Interventional Studies Biruni University permission 2017/5-1.